美國 Bio-rad轉基因儀基因槍系統Helios

顆粒轟擊用于體外和體內基因轉移的優點

簡單、快速、功能靈活的基因傳送，各種細胞類型通用
有助于瞬時表達和穩定表達
只需少量的DNA 和細胞
無需載體DNA
能進行多質粒共同導入
輸送大片段DNA
胞內目標基因可導入多個細胞
用于體內和體外轉化
沒有無關的基因或蛋白被轉導

The Helio gene gun is a convenient portable device for rapid and direct in-situ gene transfer of cells.The system USES an adjustable pulse of helium gas to drive gold particles of DNA, RNA or other biomaterials prepacked on the inside of a small plastic tube directly into a cell.The in-tube "bullet" can be easily prepared using the sample control station.

The advantages of particle bombardment for gene transfer in vitro and in vivo

simple, fast, functional gene transfer across all cell types

contributes to transient and stable expression

only small amounts of DNA and cells are needed


no carrier DNA is required

multiple plasmids can be imported together

transport large fragments of DNA

target genes can be introduced into multiple cells

for in vivo and in vitro transformation