waters SEC蛋白質(zhì)分析柱
體積排阻色譜
體積排阻（又稱凝膠過濾）色譜基于蛋白質(zhì)在溶液中的體積（流體動(dòng)力學(xué)半徑）而不是分子量對(duì)其進(jìn)行分離，體積較大的物質(zhì)會(huì)在體積較小的蛋白質(zhì)之前洗脫。這種等度洗脫技術(shù)的主要機(jī)制基于填充柱中SEC顆粒的孔徑和體積。隨著目標(biāo)蛋白質(zhì)的分子量增大，應(yīng)選用顆粒和孔徑相對(duì)更大的waters SEC蛋白質(zhì)分析柱。
與其它生物分離一樣，在這類要求極為嚴(yán)苛的應(yīng)用中，相較于HPLC色譜柱，使用粒徑更小的UPLC/UHPLC色譜柱可以在更短的時(shí)間內(nèi)實(shí)現(xiàn)優(yōu)異的分離度。
BEH顆粒技術(shù)
HPLC SEC色譜柱基于沃特世亞乙基橋雜化(BEH)顆粒技術(shù)和穩(wěn)定的二醇基鍵合技術(shù)，相較于傳統(tǒng)的100%硅膠基質(zhì)SEC產(chǎn)品，具有更的性能。
- 可對(duì)10～1500 kDa的蛋白質(zhì)進(jìn)行基于HPLC的SEC分離，且分析通量更高
- 出色的SEC色譜柱壽命，降低每次樣品分析的成本
- 出廠前經(jīng)過全面測(cè)試，可確保的色譜柱性，使得經(jīng)過驗(yàn)證的方法更加可靠
- 基于ACQUITY UPLC的SEC色譜柱產(chǎn)品，可根據(jù)應(yīng)用需求實(shí)現(xiàn)無縫方法轉(zhuǎn)換
Xbridge Protein BEH SEC蛋白分析柱
出色的蛋白質(zhì)SEC分離性能
蛋白類生物治療藥物的發(fā)現(xiàn)、研發(fā)和質(zhì)量評(píng)估通常需要使用可靠、高分離度的SEC方法。200 Å和450 Å的3.5 µm Xbridge Protein BEH SEC蛋白分析柱是對(duì)分子量約10～1500 kDa的蛋白質(zhì)進(jìn)行基于HPLC的SEC分離的理想之選。
200 Å和450 Å的XBridge Protein BEH SEC蛋白分析柱裝填二醇基鍵合的3.5 µm BEH SEC顆粒，流速和壓力耐受性優(yōu)于填充5 µm以上顆粒的硅膠基質(zhì)SEC色譜柱，因此樣品通量更高。
每個(gè)生產(chǎn)批次的200 Å和450 Å 3.5 μm Xbridge Protein BEH SEC蛋白分析柱均采用相關(guān)蛋白質(zhì)標(biāo)準(zhǔn)品進(jìn)行了全面檢測(cè)，以確保的批次間性，保證經(jīng)過驗(yàn)證的方法采集到的數(shù)據(jù)高度可信。
ACQUITY UPLC BEH SEC色譜柱
UPLC SEC色譜柱分離
ACQUITY UPLC BEH SEC色譜柱經(jīng)過專門設(shè)計(jì)，并采用BEH蛋白質(zhì)標(biāo)準(zhǔn)品進(jìn)行了QC測(cè)試，可確保出色的批次間性，提高經(jīng)過驗(yàn)證的方法的可信度。SEC系列產(chǎn)品是準(zhǔn)確測(cè)定治療性單克隆抗體中低濃度蛋白質(zhì)聚集體的理想之選，而且分析速度可達(dá)傳統(tǒng)HPLC SEC方法的十倍。相比之下（數(shù)據(jù)未顯示），BEH C4, 300Å UPLC色譜柱則能夠分離分子量、疏水性和等電點(diǎn)各異的多種蛋白質(zhì)，同時(shí)zui大限度提高回收率并減少蛋白質(zhì)殘留。
BioSuite超高分離度(UHR)色譜柱
體積排阻色譜柱（硅膠基質(zhì)）
BioSuite SEC填料的分子量排阻限值主要取決于色譜柱中硅膠基質(zhì)材料的孔徑。此外，SEC填料的粒徑和色譜柱柱長也是決定分離效率的重要參數(shù)。在分離效率方面，BioSuite UHR色譜柱（校準(zhǔn)表中未顯示），其次是BioSuite HR和標(biāo)準(zhǔn)SEC色譜柱。
Protein-Pak SEC色譜柱
Protein-Pak SEC色譜柱中填充硅膠基質(zhì)、二醇基鍵合的10 µm顆粒，提供60 Å、125 Å和300 Å三種孔徑，非常適用于分析級(jí)或?qū)嶒?yàn)室規(guī)模的生物大分子純化。
訂貨信息
| 貨號(hào) | 描述 |
| 186002164 | BioSuite SEC Column, 125Å, 5 µm, 7.8 mm X 300 mm, 5K - 150K, 1/pkg |
| 186002167 | BioSuite SEC Guard Column, 5 µm, 6 mm X 40 mm, 1/pkg |
| 186002166 | BioSuite High Resolution SEC Column, 450Å, 8 µm, 7.8 mm X 300 mm, 20K - 7M, 1/pkg |
| 186006506 | ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 300 mm, 1K - 80K, 1/pkg |
| 186002161 | BioSuite SEC Column, 125Å, 4 µm, 4.6 mm X 300 mm, 5K - 150K, 1/pkg |
| 186006505 | ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 150 mm, 1K - 80K, 1/pkg |
| 186006504 | ACQUITY UPLC Protein BEH SEC Guard Column, 125Å, 1.7 µm, 4.6 mm X 30 mm, 1K - 80K, 1/pkg |
| 186002163 | BioSuite SEC Guard Column, 4 µm, 4.6 mm X 35 mm, 1/pkg |
| 186002162 | BioSuite Ultra High Resolution SEC Column, 250Å, 4 µm, 4.6 mm X 300 mm, 10K - 500K, 1/pkg |
| 186005793 | ACQUITY UPLC Protein BEH SEC Guard Column, 200Å, 1.7 µm, 4.6 mm X 30 mm, 10K - 500K, 1/pkg |
| 186006851 | ACQUITY UPLC Protein BEH SEC Column, 450Å, 2.5 µm, 4.6 mm X 150 mm, 100K - 1500K, 1/pkg |
| 186006850 | ACQUITY UPLC Protein BEH SEC Guard Column, 450Å, 2.5 µm, 4.6 mm X 30 mm, 100K - 1500K, 1/pkg |
| 186006852 | ACQUITY UPLC Protein BEH SEC Column, 450Å, 2.5 µm, 4.6 mm X 300 mm, 100K - 1500K, 1/pkg |
| 186008471 | ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg |
| 186002168 | BioSuite SEC Column, 125Å, 10 µm, 7.5mm X 300 mm, 5K - 150K, 1/pkg |
| 186002169 | BioSuite SEC Column, 125Å, 13 µm, 21.5 mm X 300 mm, 1/pkg |
| 186005225 | ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 4.6 mm X 150 mm, 10K - 500K, 1/pkg |
| 186005226 | ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 4.6 mm X 300 mm, 10K - 500K, 1/pkg |
| 186002170 | BioSuite SEC Column, 250Å, 10 µm, 7.5mm X 300 mm, 10K - 500K, 1/pkg |
| 186002171 | BioSuite SEC Column, 250Å, 13 µm, 21.5 mm X 300 mm, 10K - 500K, 1/pkg |
| 186002172 | BioSuite SEC Column, 450Å, 13 µm, 7.5mm X 300 mm, 20k - 1M, 1/pkg |
| 186002173 | BioSuite SEC Column, 450Å, 17 µm, 21.5 mm X 300 mm, 20K - 7M, 1/pkg |
| 186002174 | BioSuite SEC Guard Column, 10 µm, 7.5mm X 75 mm, 1/pkg |
| 186002175 | BioSuite SEC Guard Column, 13 µm, 21.5 mm X 75 mm, 1/pkg |
| WAT011787 | Protein-Pak 300SW Glass Column, 10 µm, 8 x 300 mm |
| WAT085250 | Protein-Pak SEC Column, 60Å, 10 µm, 7.8 mm X 300 mm, 500 - 20K, 1/pkg |
| 176003596 | XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 300 mm, and BEH 200Å Protein Standard Mix |
| 176003595 | XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 150 mm, and BEH 200Å Protein Standard Mix |
| WAT034279 | Shodex KS-804 Column, 7 µm, 8 x 300 mm |
| WAT080013 | Protein-Pak SEC Column, 300Å, 10 µm, 7.5mm X 300 mm, 10K - 500K, 1/pkg |
| 186007637 | XBridge Protein BEH SEC Column, 125Å, 3.5 µm, 7.8 mm X 300 mm |
| WAT034276 | Shodex KS-801 Column, 7 µm, 8 x 300 mm |
| 186000926 | Protein-Pak SEC Sentry Guard Cartridge, 125Å, 10 µm, 3.9 mm X 20 mm, 2/pkg |
| 186007635 | XBridge Protein BEH SEC Guard Column, 125Å, 3.5 µm, 7.8 mm X 30 mm |
| WAT034277 | Shodex KS-802 Column, 7 µm, 8 x 300 mm |
| 176003599 | XBridge Protein BEH SEC Column, 450Å, 3.5 µm, 7.8 mm X 300 mm, and BEH450 Protein Standard Mix |
| 176003598 | XBridge Protein BEH SEC Column, 450Å, 3.5 µm, 7.8 mm X 150 mm, and BEH450 Protein Standard Mix |
| WAT025830 | Protein-Pak 60A SEC Column, 10 µm, 19x300 mm, 1/pkg |
| WAT011786 | Protein-Pak 200SW SEC Glass Column, 10 µm, 8x300 mm, 1/pkg |
| WAT025831 | Protein-Pak 125 Column, 10 µm, 19 x 300 mm |
| WAT035943 | Shodex KW-802.5 Column, 5 µm, 8 x 300 mm |
| WAT035946 | Shodex KW-803 Column, 5 µm, 8 x 300 mm |
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