NUCLEODUR® C18 Gravity

High purity silica phase for HPLC

Nonpolar high density monomeric octadecyl modification,
endcapped · 18 % C · USP L1

Properties

Key features:
Suitable for LC/MS and HPLC at pH extremes (pH 1–11)

Superior base deactivation

Ideal for method development

Technical characteristics:
High density octadecyl modification, multi-endcapped
Pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm
7, 10, 12 and 16 μm particles for preparative purposes on request
Carbon content 18 %

Recommended application:
Overall sophisticated analytical separations
Compound classes separated include pharmaceuticals, e.g., analgesics, antiinflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosuppressants

For applications click here.

不同PH下的穩定使用

圖一：色譜柱在強jian性PH=11下的穩定性

色譜柱：50×4.6mm

流動相：甲醇：水：氮=20:80:0.5（PH=11）

流 速：1.3毫升/分鐘

進樣量：2ul

溫 度：30℃

檢測器：UV 254nm

樣 品：1.茶jian Theophyline   2.kafeiyin Caffeine

圖二：色譜柱在強酸性PH=1.5下的穩定性

色譜柱：NUCLEODUR(閃電) C18 Gravity, 125×4.0mm 5um

流動相：乙腈：1% TFA水溶液=50:50（PH=1.5）

流 速：1.0毫升/分鐘

進樣量：5ul

溫 度：30℃

檢測器：UV 230nm

樣 品：1. 吡啶 2.甲苯 3.乙苯

   圖一是NUCLEODUR(閃電) C18 Gravity與其他4個品牌新型反相C18色譜柱穩定性的比較。在PH=11的流動條件下，連續300次進樣后，NUCLEODUR(閃電) C18 Gravity沒有出現因柱效下降，造成的出峰展寬或保留時間縮短的現象。

   圖二是 NUCLEODUR(閃電) C18 Gravity系列反相色譜柱在低PH條件下優異的穩定性。由于硅膠表面特別穩定的鍵合，有效防止了鍵合相在低PH時的脫落，即使經過在5000ml的PH=1.5流動相沖洗后，3種被測樣品的保留時間沒有變化。

所以在NUCLEODUR(閃電) C18 Gravity系列可以在PH=1.0—11.0之間穩定使用，是您研究開發特別條件應用的好幫手。

通痹靈中maqianzijian，士的ning的測定

色譜柱：NUCLEODUR(閃電) C18 Gravity 125×4.6mm 5um

流動相：甲醇：水：三乙胺=60:40:0.5 使用冰醋酸調整PH=5

流 速：1.0 mL/min

檢測器：UV 254nm

回收率：maqianzijian，shidening分離度大于3   maqianzijian的線性r=0.9996，回收率=97.93%

       shidening的線性r=0.9996，回收率=97.69%

人血漿中的測定

色譜柱：NUCLEODUR(閃電) C18 Gravity 250×4.6mm 5um

流動相：甲醇：0.02mol/L 醋酸銨（PH=9.9）=92:8

流 速：1.0 mL/min

檢測器：UV 254nm

回收率：，內標分離度大于3   的線性r=0.9999

FMOC—衍生檢測草甘膦，AMPA和草銨膦

色譜柱：㏄250×3mm NUCLEODUR C18 Gravity，5um

衍 生：加入洗脫液0.1ml緩沖液，PH9.5

緩沖液的配置:
             a)19.108 g Na2B4O7 10H2O/1000 ml water

             b)12.404 g H2BO2 and 2.92g NaCI/1000 ml water

             a)和b)體積比1:1混合，使用20%NaOH調節PH9.5加入 1.0mlFMOC—CI(2.5 m mol乙腈)，反應時間：30—60分鐘，加入3毫升yimi震蕩1分鐘，取1毫升水相，加入30ul 130% HCI 震蕩1—2小時。

色譜條件：流動相：A)乙腈； B)H3PO4,PH1.2

                  30—35% A in 27 min,35—90% A in 3 min,90% A for 6 min,90—30% A in 2 min,30% A for 7 min

流 速：0.5 ml/min

進樣量：100 or 10 ul

溫 度：30℃

檢測器：Fluorescence,Ex.263 nm,Em.317 nm

檢出限：0.05 ug/l

樣 品：glyphosate 草甘膦    9.825 min

            AMPA                       13.633min

            Glufosinate草銨膦   15.675min

作 者：Schussier;Mikler

來 源：Bayrisches Landesamtfur Wasserwirtschaft,Munchen.Germany

對照品的標定

使用NUCLEODUR C18 Gravity和對照柱X按照《中國藥典》方法檢測進行標定

色譜柱：NUCLEODUR C18 Gravity 250×4.6mm 5um  對照柱X 250×4.6mm 5um

流動相：0.005 mol/L 四丁基氫氧化銨（PH=5.0）:乙腈=75:25

溫  度：35℃

檢測器：UV 230nm

結果:

EC standard columns for analytical HPLC

Ordering information

Eluent in column acetonitrile – water

Length ->

30 mm

50 mm

75 mm

100 mm

125 mm

150 mm

250 mm

NUCLEODUR® C18 Gravity, 1.8 µm

2 mm ID

760078.20

760079.20

760071.20

760076.20

760075.20

3 mm ID

760078.30

760079.30

760076.30

4 mm ID

760078.40

760079.40

760076.40

4.6 mm ID

760078.46

760079.46

760076.46

EC guard columns

4 x 2 mm

761901.20

4 x 3 mm

761901.30

NUCLEODUR® C18 Gravity, 3 µm

2 mm ID

760080.20

760084.20

760081.20

760083.20

760082.20

3 mm ID

760080.30

760084.30

760081.30

760083.30

760082.30

4 mm ID

760080.40

760084.40

760081.40

760083.40

760082.40

4.6 mm ID

760080.46

760086.46

760084.46

760081.46

760083.46

760082.46

EC guard columns

4 x 2 mm

761902.20

4 x 3 mm

761902.30

CC guard columns

8 x 3 mm

761124.30

8 x 4 mm

761124.40

NUCLEODUR® C18 Gravity, 5 µm

2 mm ID

760102.20

760104.20

760100.20

760103.20

760101.20

3 mm ID

760102.30

760104.30

760100.30

760103.30

760101.30

4 mm ID

760102.40

760104.40

760100.40

760103.40

760101.40

4.6 mm ID

760102.46

760106.46

760104.46

760100.46

760103.46

760101.46

EC guard columns

4 x 2 mm

761903.20

4 x 3 mm

761903.30

CC guard columns

8 x 3 mm

761125.30

8 x 4 mm

761125.40

Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns

EC columns with ID

Use guard
column holder

2 mm

3 mm

4 mm

4.6 mm

Column Protection System

EC guard column

4/2

4/3

4/3

4/3

REF 718966

ChromCart® guard column system

CC guard column

8/3

8/3

8/4

8/4

REF 721359

Custom-packed EC columns with other dimensions are available on request.

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VarioPrep columns for preparative HPLC

Ordering information

Eluent in column acetonitrile – water

Length

10 mm ID

21 mm ID

32 mm ID

40 mm ID

NUCLEODUR® C18 Gravity, 5 µm

50 mm

762103.100

762103.210

125 mm

762109.100

762109.210

150 mm

762100.400

250 mm

762113.100

762113.210

762113.320

762113.400

NUCLEODUR® C18 Gravity, 10 µm

250 mm

762250.210

762250.400

VP guard columns with NUCLEODUR® C18 Gravity

8 mm ID

16 mm ID

32 mm ID

10 mm

762160.80

762160.160

15 mm

762163.320

VP guard column

Size

Pack of

for VP column

required holder

REF

VP 10/8

10 x 8 mm ID

2

10 mm ID

8 mm

718251

VP 10/16

10 x 16 mm ID

2

21 mm ID

16 mm

718256

VP 15/32

15 x 32 mm ID

1

32–40 mm ID

32 mm

718253

Custom-packed VP columns with other dimensions are available on request.

For details of the VarioPrep column system click here.

Standard program of columns packed with NUCLEODUR® C18 Gravity

Column system

Particle size:

1.8 µm

3 µm

5 µm

10 µm

Analytical

EC columns (standard system, 2–4.6 mm)

Preparative

VarioPrep columns (VP, ≥ 8 mm)

  if you do not know our HPLC column systems, click the "i" for further information

Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.