NUCLEOSIL® SiOH

Unmodified silica phases for HPLC · USP L3

Features

Spherical silica, pH stability at 20 °C: 2–8
Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å
Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
Separation mechanism based on polar interactions

Physical properties of unmodified NUCLEOSIL® SiOH materials

Phase

Pore size

Pore volume

Surface (BET)

Density

Pressure stability

NUCLEOSIL 50

50 Å

0.8 mL/g

420 m2/g

0.45 g/mL

500 bar

NUCLEOSIL 100

100 Å

1 mL/g

350 m2/g

0.36 g/mL

500 bar

NUCLEOSIL 120

120 Å

0.65 mL/g

200 m2/g

0.55 g/mL

500 bar

NUCLEOSIL 300

300 Å

0.8 mL/g

100 m2/g

0.45 g/mL

400 bar

NUCLEOSIL 500

500 Å

0.8 mL/g

35 m2/g

0.45 g/mL

400 bar

NUCLEOSIL 1000

1000 Å

0.8 mL/g

25 m2/g

0.45 g/mL

300 bar

NUCLEOSIL 4000

4000 Å

0.7 mL/g

10 m2/g

0.48 g/mL

300 bar

Maximum packing pressure; the maximum working pressure for the EC columns listed below is 400 bar.

EC standard columns for analytical HPLC

Ordering information

Eluent in column is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the columns with THF first.

Length 250 mm

EC guard columns

CC guard columns

NUCLEOSIL® 50-5 · particle size 5 µm, pore size 50 Å

4.6 mm ID

720093.46

721167.30

721600.40

NUCLEOSIL® 100-5 · particle size 5 µm, pore size 100 Å

4.6 mm ID

720099.46

721518.30

721872.40

Columns / pack

1

3

3

EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
CC 8/4 guard column cartridges are used for EC columns of 4.6 mm ID with guard column adapter EC (REF 721359).

Custom-packed EC columns with other dimensions are available on request.

Unmodified NUCLEOSIL® SiOH bulk packingsOrdering information

Phase

Pore size

Particle size

Pack of 10 g

Pack of 100 g

NUCLEOSIL 50-3

50 Å

3 µm

712000.10

712000.100

NUCLEOSIL 50-5

50 Å

5 µm

712010.10

712010.100

NUCLEOSIL 50-7

50 Å

7 µm

712020.10

712020.100

NUCLEOSIL 50-10

50 Å

10 µm

712030.10

712030.100

NUCLEOSIL 100-3

100 Å

3 µm

712360.10

712360.100

NUCLEOSIL 100-5

100 Å

5 µm

712040.10

712040.100

NUCLEOSIL 100-7

100 Å

7 µm

712050.10

712050.100

NUCLEOSIL 100-10

100 Å

10 µm

712060.10

712060.100

NUCLEOSIL 120-3

120 Å

3 µm

712390.10

719390.100

NUCLEOSIL 120-5

120 Å

5 µm

712400.10

712400.100

NUCLEOSIL 120-7

120 Å

7 µm

712410.10

712410.100

NUCLEOSIL 120-10

120 Å

10 µm

712420.10

712420.100

NUCLEOSIL 300-5

300 Å

5 µm

712430.10

712430.100

NUCLEOSIL 300-7

300 Å

7 µm

712440.10

712440.100

NUCLEOSIL 300-10

300 Å

10 µm

712450.10

712450.100

NUCLEOSIL 500-5

500 Å

5 µm

712680.10

712680.100

NUCLEOSIL 500-7

500 Å

7 µm

712690.10

712690.100

NUCLEOSIL 500-10

500 Å

10 µm

712700.10

712700.100

NUCLEOSIL 1000-5

1000 Å

5 µm

712710.10

712710.100

NUCLEOSIL 1000-7

1000 Å

7 µm

712720.10

712720.100

NUCLEOSIL 1000-10

1000 Å

10 µm

712730.10

712730.100

NUCLEOSIL 4000-5

4000 Å

5 µm

712850.10

712850.100

NUCLEOSIL 4000-7

4000 Å

7 µm

712860.10

712860.100

NUCLEOSIL 4000-10

4000 Å

10 µm

712870.10

712870.100