NUCLEODUR® C18 Isis

High purity silica phase for HPLC

Nonpolar crosslinked polymeric octadecyl modification
high steric selectivity · endcapped · 20 % C · USP L1

Properties

Key features:
Exceptional steric selectivity

Outstanding surface deactivation

Suitable for LC/MS and HPLC at pH extremes (pH 1–10)

Technical characteristics:
C18 phase with special polymeric, crosslinked surface modification
pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm;
carbon content 20 %

Recommended application:
Steroids, (o,p,m-) substituted aromatics, fat-soluble vitamins
For applications click here.

鰻魚中九種磺胺殘留檢測

色譜柱：EC 250×4.6mm NUCLEODUR C18 ISIS,5um
流動相：乙腈：0.3% 醋酸水溶液     梯度洗脫
流  速：0.8毫升/分鐘
進樣量：20 ul
溫  度：35℃
檢測器：UV 270nm
混標濃度：1.0 ug/mL
樣  品：1.SG—磺胺胍  2.SD—  3.SM2—  4.SMM—磺胺甲氧嗪  5.SCP—磺胺氧噠嗪  7.SMP—  8.SMZ—  9.SDM—磺胺二甲氧嘧啶  10.SQ—磺胺喹噁啉

色譜柱：NUCLEODUR C18 ISIS,5um 125×2mm
流動相：乙腈：水=100:5
流  速：0.2毫升/分鐘
進樣量：5 ul
柱  溫：25℃
檢測器：UV 275nm
樣  品：1.維生素A  2.維生素A乙酯  3.維生素K2  4.維生素D2  5.維生素D3    6.r-生育酚  7.a-生育酚  8.a-生育酚乙酯  9.維生素K1

藥物分析

 

色譜柱：NUCLEODUR C18 ISIS,5um 250×4.6mm
流動相：乙腈：水=30:70
流  速：1.0毫升/分鐘
進樣量：5 ul
溫  度：25℃
檢測器：UV 260nm
樣  品：1.  2.

胺類樣品的檢測

 

色譜柱：EC 150×4.6mm NUCLEODUR C18 ISIS,5um
流動相：乙腈：50mM磷酸二氫鉀（PH=8）=40:6
流  速：1.0毫升/分鐘
進樣量：8 ul
溫  度：25℃
檢測器：UV 254nm樣  品：1.  2.吡啶  3.脫乙基阿特拉津  4.4-乙酰基吡啶  5.4-乙基*

 6.N.N-二jiajibenan  7.4-氨基蒽醍  8.3,5-二硝基-1-苯基乙基*

EC standard columns for analytical HPLC

Ordering information

Eluent in column acetonitrile – water

Length ->

30 mm

50 mm

75 mm

100 mm

125 mm

150 mm

250 mm

NUCLEODUR® C18 Isis, 1.8 µm

2 mm ID

760406.20

760405.20

760396.20

760407.20

760409.20

3 mm ID

760406.30

760405.30

760407.30

4 mm ID

760406.40

760405.40

760407.40

4.6 mm ID

760406.46

760405.46

760407.46

EC guard columns:

4 x 2 mm

761910.20

4 x 3 mm

761910.30

NUCLEODUR® C18 Isis, 3 µm

2 mm ID

760400.20

760401.20

760402.20

760403.20

760404.20

3 mm ID

760400.30

760401.30

760402.30

760403.30

760404.30

4 mm ID

760400.40

760401.40

760402.40

760403.40

760404.40

4.6 mm ID

760400.46

760397.46

760401.46

760402.46

760403.46

760404.46

EC guard columns:

4 x 2 mm

761911.20

4 x 3 mm

761911.30

CC guard columns:

8 x 3 mm

761300.30

8 x 4 mm

761300.40

NUCLEODUR® C18 Isis, 5 µm

2 mm ID

760410.20

760415.20

760412.20

760413.20

760414.20

3 mm ID

760410.30

760415.30

760412.30

760413.30

760414.30

4 mm ID

760410.40

760415.40

760412.40

760413.40

760414.40

4.6 mm ID

760410.46

760416.46

760415.46

760412.46

760413.46

760414.46

EC guard columns:

4 x 2 mm

761912.20

4 x 3 mm

761912.30

CC guard columns:

8 x 3 mm

761310.30

8 x 4 mm

761310.40

Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns

EC columns with ID

Use guard
column holder

2 mm

3 mm

4 mm

4.6 mm

Column Protection System

EC guard column

4/2

4/3

4/3

4/3

REF 718966

ChromCart® guard column system

CC guard column

8/3

8/3

8/4

8/4

REF 721359

Custom-packed EC columns with other dimensions are available on request.

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VarioPrep columns for preparative HPLC

Ordering information

Eluent in column acetonitrile – water

Length

10 mm ID

21 mm ID

32 mm ID

40 mm ID

NUCLEODUR® C18 Isis, 5 µm

50 mm

762404.100

762404.210

125 mm

762405.100

762405.210

150 mm

762406.400

250 mm

762403.100

762403.210

762403.320

762403.400

VP guard columns with NUCLEODUR® C18 Isis

8 mm ID

16 mm ID

32 mm ID

10 mm

762420.80

762420.160

15 mm

762422.320

VP guard column

Size

Pack of

for VP column

required holder

REF

VP 10/8

10 x 8 mm ID

2

10 mm ID

8 mm

718251

VP 10/16

10 x 16 mm ID

2

21 mm ID

16 mm

718256

VP 15/32

15 x 32 mm ID

1

32–40 mm ID

32 mm

718253

Custom-packed VP columns with other dimensions are available on request.

For details of the VarioPrep column system click here.

Standard program of columns packed with NUCLEODUR® C18 Isis

Column system

Particle size:

1.8 µm

3 µm

5 µm

Analytical

EC columns (standard system, 2–4.6 mm)

Preparative

VarioPrep columns (VP, ≥ 8 mm)

 if you do not know our HPLC column systems, click the "i" for further information

Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.